TY - JOUR TI - Impact of stationary-phase pore size on chromatographic performance using oligonucleotide separation as a model AU - Bagge, Joakim AU - Enmark, Martin AU - Leśko, Marek AU - Limé, Fredrik AU - Fornstedt, Torgny AU - Samuelsson, Jörgen T2 - Journal of Chromatography A AB - A combined experimental and theoretical study was performed to understand how the pore size of packing materials with pores 60–300 Å in size affects the separation of 5–50-mer oligonucleotides. For this purpose, we developed a model in which the solutes were described as thin rods to estimate the accessible surface area of the solute as a function of the pore size and solute size. First, an analytical investigation was conducted in which we found that the selectivity increased by a factor of 2.5 when separating 5- and 15-mer oligonucleotides using packing with 300 Å rather than 100 Å pores. We complemented the analytical investigation by theoretically demonstrating how the selectivity is dependent on the column's accessible surface area as a function of solute size. In the preparative investigation, we determined adsorption isotherms for oligonucleotides using the inverse method for separations of a 9- and a 10-mer. We found that preparative columns with a 60 Å-pore-size packing material provided a 10% increase in productivity as compared with a 300 Å packing material, although the surface area of the 60 Å packing is as much as five time larger. DA - 2020/12/20/ PY - 2020 DO - 10.1016/j.chroma.2020.461653 DP - ScienceDirect VL - 1634 SP - 461653 J2 - Journal of Chromatography A LA - en SN - 0021-9673 UR - http://www.sciencedirect.com/science/article/pii/S0021967320309274 Y2 - 2020/11/08/14:43:03 KW - Adsorption isotherm KW - Gradient elution KW - Ion-pair RPLC KW - Oligonucleotides KW - Pore size KW - Surface area ER -