TY - JOUR TI - Systematic investigations of peak deformations due to co-solvent adsorption in preparative supercritical fluid chromatography AU - Glenne, Emelie AU - Leek, Hanna AU - Klarqvist, Magnus AU - Samuelsson, Jörgen AU - Fornstedt, Torgny T2 - Journal of Chromatography A AB - Strangely shaped overloaded bands were recently reported using a standard supercritical fluid chromatographic system comprising a diol column as the stationary phase and carbon dioxide with methanol as the mobile phase. Some of these overloaded elution profiles appeared strongly deformed and even had “anti-Langmuirian” shapes although their solute compounds had “Langmuirian” adsorption. To obtain a more complete understanding of the generality of these effects, the investigation was expanded to cover also other common co-solvents, such as ethanol, 2-propanol, and acetonitrile, as well as various stationary phase materials, such as silica, and 2-ethylpyridine. From this expanded study it could be confirmed that the effects of deformed overloaded solute band shapes, due to co-solvent adsorption, is general phenomena in supercritical fluid chromatographic. It could also be concluded that these effects as well as previously observed “solvent effects” or “plug effects” are entirely due to competition between the solute and solvent molecules for the adsorption sites on the stationary phase surface. Finally, guidelines were given for how to evaluate the risk of deformations occurring for a given solvent–column combination, based simply on testing retention times of solutes and co-solvent. DA - 2017/05/05/ PY - 2017 DO - 10.1016/j.chroma.2017.03.053 DP - ScienceDirect VL - 1496 SP - 141 EP - 149 J2 - Journal of Chromatography A SN - 0021-9673 UR - http://www.sciencedirect.com/science/article/pii/S0021967317304326 KW - Adsorption strength KW - Anti-Langmuir band shape KW - Co-solvent adsorption KW - Langmuir band shape KW - Supercritical fluid chromatography ER -