TY - JOUR TI - Evaluation of a combined linear–nonlinear approach for column characterization using modern alkaline-stable columns as model AU - Undin, Torgny AU - Samuelsson, Jörgen AU - Törncrona, Anders AU - Fornstedt, Torgny T2 - Journal of Separation Science AB - This study investigates if deeper understanding is achieved when combining nonlinear and linear chromatographic column characterization methods. As test systems, two hybrid columns (Phenomenex Gemini-NX C18 and Kromasil Eternity C18) and one classic one (Kromasil-C18) were selected. The nonlinear methods were based on firm adsorption theory and involved determination of adsorption isotherms followed by calculations with a new numerical tool, adsorption energy distribution, on probe components at different pH values. The linear methods involved the hydrophobic subtraction model and selected probe components retention factors as a function of pH. The combined analysis indicated that both complementary and confirmative information can be achieved regarding the actual model systems. DA - 2013/// PY - 2013 DO - 10.1002/jssc.201201132 DP - Wiley Online Library VL - 36 IS - 11 SP - 1753 EP - 1761 J2 - J. Sep. Sci. LA - en SN - 1615-9314 UR - http://onlinelibrary.wiley.com/doi/10.1002/jssc.201201132/abstract Y2 - 2013/08/29/10:37:15 KW - Adsorption isotherms KW - Characterization KW - Linear methods KW - Nonlinear methods KW - Retention ER -