TY - JOUR TI - Exogenous factors contributing to column bed heterogeneity: Part 1: Consequences of ‘air’ injections in liquid chromatography AU - Samuelsson, Jörgen AU - Fornstedt, Torgny AU - Shalliker, Andrew T2 - Journal of Chromatography A AB - It has been shown that not only the packing homogeneity, but also factors external to the column bed, such as, frits and distributors can have important effects on the column performance. This current communication is the first in a series focusing on the impact of exogenous factors on the column bed heterogeneity. This study is based on several observations by us and others that chromatographic runs often, for technical reasons, include more or less portions of air in the injections. It is therefore extremely important to find out the impact of air on the column performance, the reliability of the results derived from analyses where air was injected, and the effect on the column homogeneity. We used a photographic approach for visualising the air transport phenomena, and found that the air transport through the column is comprised of many different types of transport phenomena, such as laminal flow, viscous fingering like flows, channels and bulbs, and pulsations. More particularly, the air clouds within the column definitely interact in the adsorption, i.e. mobile phase adsorbed to the column surface is displaced. In addition, irrespective of the type of air transport phenomena, the air does not penetrate the column homogeneously. This process is strongly flow dependent. In this work we study air transport both in an analytical scale and a semi-prep column. DA - 2015/08/07/ PY - 2015 DO - 10.1016/j.chroma.2015.06.016 DP - ScienceDirect VL - 1406 SP - 186 EP - 191 J2 - Journal of Chromatography A SN - 0021-9673 ST - Exogenous factors contributing to column bed heterogeneity UR - http://www.sciencedirect.com/science/article/pii/S0021967315008638 Y2 - 2018/11/22/07:50:14 KW - Air injection KW - Chromatography KW - Column bed heterogeneity KW - Gas transport ER -